Skip to content

Identification & Background:

Project title: Bio4Drinks –  Obtaining Multifunctional Natural Ingredients for the Beverage Industry

Project code: NORTE-01-0247-FEDER-113508

Main objective: Strengthening research, technological development, and innovation

Region of intervention: North

Beneficiary entities:

Tree Flowers Solutions, Lda (Project Leader)

MORE – Laboratório Colaborativo Montanhas de Investigação – Associação

IPB – Instituto Politécnico de Bragança

Fermentum – Engenharia das fermentações, Lda

Approval date: 29-07-2021

Start date: 01-09-2021

Conclusion date: 30-06-2023

Total eligible investment: 615.438,01 €

Financial support of the European Union: FEDER – 458.150,90€



1) Valorization of at least two by-products of the wine sector (seeds, leaves, grape pomace) and two of the chestnut (flowers, leaves, hedgehogs, chestnut shell), in bioactive ingredients of high economic value (production of extracts);

2) Develop a sustainable process in terms of cost-effectiveness and low environmental impact to obtain 4 extracts of wine and nut by-products, rich in bioactive phenolic compounds;

3) Evaluate and characterize the phenolic extracts, in terms of stability, bioaccessibility and bioavailability;

4) Develop optimized formulations of extracts, aiming to stabilize the phenolic compounds for application in two drinks, beer and flavoured water;

5) Validate, on a pilot scale, the effectiveness of extracts rich in polyphenols applied in the food prototypes developed, as antimicrobial and antioxidant agents, in order to act as a preservative in the case of beers and as a functionalizing agent in the case of flavoured waters.




  1. Technical specifications;
  2. In vitro studies of bioactive properties and toxicity of extracts;
  3. Stability studies of the developed ingredients and beverages;
  4. Obtaining beverage prototypes with the incorporation of the studied ingredients;
  5. Dissemination, communication and exploitation of project results;
  6.  Project management.

Expected results

Get at least 4 new products, namely:

  1. Two new craft beers with lower risk of oxidation and organoleptic alterations (reduction to 3-4% of loss of organoleptic characteristics after 4 months of storage). It should be noted that, on average, a craft beer has a shelf life of 10 months, but after 4 months of storage, it loses between 30 and 40% of its organoleptic characteristics. In this sense, Bio4Drinks aims to reduce these changes by about 90%.
  2. Two flavoured waters with functional properties (e.g. antioxidant), contributing to the improvement of existing products.

Avenida Cidade de Léon, 506
5301-358 Bragança

+351 300 081 998
+351 300 081 999

Cofinanciado por:

Barra Financiamento Colabs